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Differentiation was confirmed by alizarin red staining, which detects mineralized matrix deposition, and oil red O staining, which identifies lipid vacuoles in adipocytes

The market size was approximately $120-170 million in 2024 and is expected to grow to $250-500 million by 2032-2033, with a compound annual growth rate as high as 9.5%-11.5%

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doi: 10.1038/nm.2123 131

Beginning with a smaller dose and gradually increasing is the usual method, allowing the digestive system to acclimate to the dense nutritional content of spirulina without causing gastrointestinal distress