Human microsomal prostaglandin E synthase 1: a member of the MAPEG protein superfamily
The Tohoku Hospital Pediatrics-1 (THP-1) cell line was purchased from the China Center for Type Culture Collection and cultured in THP-1specific complete medium (RPMI-1640 + 10%FBS + 0.05 mM-mercaptoethanol + 1% penicillin, CM-0233, Procell Life Science & Technology, China)
After 24 h of IGF1 stimulation, the LDH activity did not change in U87Empty cells but had a significant increase in WT and mutant clones that were abrogated with OSI906 (data not shown)

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