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Cellular and molecular methods Cell culture MEFs were cultured in standard DMEM medium with 10% FBS (Gibco, LifeTechnologies, 10270106) with antibiotics (100 U ml 1 penicillinstreptomycin

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Fragment 176-191 has been heavily researched in animal models because even while it preserves the fat-burning effects of hGH, it avoids some other effects of its parent protein such as increasing insulin-like growth factor-1 (IGF-1) levels, negatively impacting carbohydrate metabolism, altering insulin sensitivity, increasing long bone growth, and so forth

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