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Price: 27.70 USD
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doi: 10.1017/S0007114514000609 124 OlivaresMLaparraMSanzY

His-tagged proteins and enzymes can be purified using immobilized-metal affinity chromatography (IMAC), which exploits the strong co-ordinate bonds formed the his-tag and the transition metal ions in the support (or stationary phase), using Ni columns to trap the desired protein 13,14,15,16

Battula V, Samudio I, Konopleva M, Andreeff M

New insights into the structure and function of the pseudokinase domain in JAK2

Funding This work was supported by the Natural Science Foundation of Hebei Province (C2022205026), the Foundation of Hebei Normal University (L2023T05), and the National Natural Science Foundation of China (31472050)