10.1038/s41569-024-01062-6 30 CarliniN
The candidates selected are predicted to be orally bioavailable according to the analysis of Lipinski et al
Can the CellTiter-Glo 3D Assay be used with tissues or non-3D samples (pellets, suspensions, tissue material)
Notably, L-carnitine levels, particularly extracellular levels, were significantly elevated in macrophages with an M2 phenotype (Fig
13C, 15N, 2H (D), 18O labeling Tailor-made small molecules, amino acids, peptides, nucleotides High chemical and isotopic purity ( 98%) Scalability from milligram to gram quantities Fast turnaround and stringent QC documentation SILAC media preparation with isotopically labeled amino acids (13C6-Arg, 13C6-Lys, etc.) Complete experimental setup, including: Label incorporation monitoring Cell viability and growth optimization Proteomic sample preparation (trypsin digestion, desalting) Data acquisition-ready samples for LC-MS/MS 13C- or 15N-based tracer studies Time-course metabolic flux analysis (MFA) Labeling in bacteria, yeast, mammalian cells, and plant systems Support for downstream GC-MS, LC-MS, and NMR analysis Quantitative pathway modeling and interpretation available Uniform or site-specific 13C, 15N, and 2H labeling of proteins and peptides Recombinant protein expression in labeled media Sample preparation for solution-state or solid-state NMR LC-MS/MS analysis of labeled samples Isotopomer distribution analysis Quality control and purity assessment of labeled compounds Interpretation and visualization of isotopic labeling patterns We perform metabolic stability analysis using stable isotope labeling, a crucial method for assessing compound metabolic stability and initiating enzyme effect studies
