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The assays were performed using an AB Sciex 4000 QTrap system (AB Sciex, Framingham, MA, USA)

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After washing with PBS-Tween 20, slides were immersed in Strep-HRP solution and counterstained with methyl green

Regeno Blend (BPC-157, TB-500, Cartalax) 30mg REGENO Cartalax + TB-500 + BPC-157 is a triple-peptide research formulation utilized in preclinical research involving tissue-associated signaling, extracellular matrix organization, cellular migration dynamics, and cartilage-related regulatory pathways