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Immunity 44, 380390, doi: 10.1016/j.immuni.2016.01.021 (2016)

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10.1111/j.1365-2672.2006.02904.x

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Liu J, Zhang J, Song K, Du J, Wang X, Liu J, Li B, Ouyang R, Miao Y, Sun Y, et al

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Under normoxic conditions, vitamin C-dependent prolyl hydroxylase enzymes hydroxylate HIF-1, marking it for proteasomal degradation

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The secondary anti-rabbit antibody coupled with horseradish peroxidase ensured a readout of 450 nm after addition of the reagent

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