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Finally, the samples were brought to dryness using an evaporation system (TurboVap LV, Biotage, Uppsala, Sweden) and resuspended in 100 L methanol-water (50/50, v/v) for UPLC-MS/MS automated injections

Thanks to its design, Semax effectively passes through the blood-brain barrier (BBB), exerting its nootropic benefits without any known endocrine effects [1, 2, 3, 4, 5]

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Determining the correct dosage, method of administration, and length of each treatment was a steep learning curve