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Add 300 L complete medium and 300 L JC-10 staining working solution, mix well and incubate in a incubator for 20 min, then discard the working solution and wash twice with 1x JC-10 staining buffer, followed by 600 L cell culture medium

PRP is short for Platelet Rich Plasma

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Run a peptide reconstitution calculator: input molecular weight, mass on the label, target concentration, and target final volume to determine the precise water volume needed