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The reactions were incubated at 25 C for 20 min to allow telomerase to synthesise the TRAP ladders, then the qPCR was carried out at 95 C for 10 min and 35 cycles of 95 for 30 s, and 60 for 90 s

390 /Investigators/ examined the efficacy of tetrasodium EDTA in eradicating biofilms derived from salivary inocula or pure cultures of Candida albicans on discs of polymethyl methacrylate (PMMA) denture base or on toothbrushes that had been used normally for 4-8 weeks

2-(new ) Aqua (Water), Octocrylene, Propanediol, Butyl Methoxydibenzoylmethane, Ethylhexyl Salicylate, Polymethyl Methacrylate, Dimethicone, Phenylbenzimidazole Sulfonic Acid, Polysilicone-15, Propylene Glycol Dicaprylate/Dicaprate, , Bis-Ethylhexyloxyphenol Methoxyphenyl Triazine, Silica, Dimethicone/Vinyl Dimethicone Crosspolymer, 1,2-Hexanediol, Hydroxyacetophenone, Polysorbate 60, PEG-10 Dimethicone, Sodium Hyaluronate, Tocopheryl Acetate, Xanthan Gum, Caprylyl Glycol, Parfum (Fragrance), Disodium EDTA, PEG-8, Tocopherol, Tropolone, Ascorbyl Palmitate, Ascorbic Acid, Citric Acid