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The obtained Cy5.5-labelled TP-10@CaP-CTP and TP-10@CaP nanoparticles were collected by centrifugation, washed with deionized water and ethanol, and dispersed in PBS for further in vivo imaging

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CD44 deletion was achieved by precomplexing two Cd44 single guide RNA (sgRNA) guides (300 pmoles of Cd44 sgRNA1 plus 300 pmoles of Cd44 sgRNA2) with 36.3 pmoles of Cas9 protein (IDT, 1081059)