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To further delineate which histone modification is critical in mediating the effect of glutamine supplementation, we treated cells cultured in high glutamine with JIB04 (a pan demethylase inhibitor), PBIT (an H3K4me3 demethylase inhibitor), or GSKJ4 (an H3K27me3 demethylase inhibitor) to determine whether these histone marks are necessary for the effect of high glutamine 33,34,35