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Similarly, RWPE-1 cells were seeded at a density of 1 105 cells per well in 6-well K-SFM plates containing 10% fetal bovine serum and 1% penicillinstreptomycin

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By using affinity chromatography, one can separate proteins that bind to a certain fragment from proteins that do not bind that specific fragment.Because this technique of purification relies on the biological properties of the protein needed, it is a useful technique and proteins can be filtered multiple folds in a single step

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