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For the rst method, approximately 0.02 g of fresh plant material was extracted directly after harvest with Fast Prep, 60 s, 6.0 m/s (Fast Prep-24, MP Biomedicals, Fisher Scientic GmbH, Schwerte) in 2.0 mL screw-top microtubes (Sarstedt, Taufkirchen, Germany) containing 0.2 g 1.4 mm ceramic beads and six 2.8 mm ceramic beads (Preqlab, VWR, Darmstadt, Germany) for grinding, and 1 mL ice-cold 50 mM phosphate buer pH 7 containing 1% PVP40 and 0.2 mM EDTA as extraction medium

Pinch a 2 cm skin fold and insert the needle at a 4590 angle

PMID 41966639

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Mar Biol 137:453461 Regoli F, Benedetti M, Krell A, Abele D (2012) Oxidative challenges in polar seas