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Gibco) and the R28 cells were incubated in an anoxic environment (95% N 2 , 5% CO 2 ) for 2 h

The PCR conditions consisted of denaturation at 95C for 3 min, followed by 40 cycles of denaturation at 95C for 30 s, annealing at 58C for 30 s, and extension at 72C for 30 s

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