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Description

Standard or sample was transferred in triplicate to a 96-well plate and GSH was then derivitized by the addition of 10 mM 2,3-naphthalenedicarboxyaldehyde and the fluorescence at Ex/Em wavelength of 472/528 nm was quantified using a fluorescent plate reader (Synergy 2, BioTek)

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Hypertens (Dallas Tex: 1979)

However, new insights into Maillard-type chemistry and the function of myeloperoxidase have led to the recognition of methionine and threonine as precursors of acrolein [6, 7]