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16 hr half life desipramine (generic, Norpramin ) is an active metabolite of imipramine that selectively inhibits NET, with minor effects on SERT

Mol Cell (2019) 73:35463.e3

coli strains used originated or were derived from the KEIO collection (Baba et al., 2006), and were routinely cultivated at 37 C in Luria-Bertani (LB) medium, supplemented with antibiotics when required for plasmid maintenance and marker selection (ampicillin 200 g/mL or kanamycin 100 g/mL), if not stated differently

MAT1A is expressed mainly in liver cells and encodes the catalytic subunit 1 (MAT1a), which forms MATI (homo-tetramer) and MATIII (homo-dimer) ( MAT2A and MAT2B are expressed in all tissues, encoding the catalytic subunit 2 (MAT2a) and the regulatory subunit (MAT2b), respectively ( MAT2A while downregulating the expression of MAT2B ( Figure 1 )

Funding This work was funded by the Natural Sciences and Engineering Research Council of Canada (NSERC, Discovery grant nos