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S.ZhangZ.et al (2021)

10.1186/s13321-018-0285-8 38 KuhnM.von MeringC.CampillosM.JensenL

10.1016/j.tice.2016.12.004 58 HartvigsenJ.HancockM

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Western blot analysis involved preparing whole cell lysates using RIPA lysis buffer, quantifying protein concentration via BCA assay, followed by SDS-PAGE, transfer to nitrocellulose membranes, incubation with primary and HRP-conjugated secondary antibodies, and detection using an ECL solution [1]