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2.4 Muscle proteome Frozen gastrocnemius tissue from the right hindlimb was crushed with a pestle and placed in homogenization buffer (1% Triton X-100, 50 mM HEPES, 12 mM Na pyrophosphate, 100 mM NaF, 10 mM EDTA, protease inhibitor, phosphatase inhibitor) with a stainless-steel bead

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Die verschiedenen erfindungemssen Salze knnen entweder direkt aus der Verbindung der Formel 3a (Methode A) oder mittels der Reaktion eines Erdalkalimetallsalzes, beispielsweise das Kalziumsalz der Formel 3i , mit dem Oxalsuresalz des entsprechenden Kations oder Amins (Methode B) hergestellt werden.The various salts according to the invention can be prepared either directly from the compound of the formula 3a (method A) or by means of the reaction of an alkaline earth metal salt, for example the calcium salt of the formula 3i, with the oxalic acid salt of the corresponding cation or amine (method B)