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The first is conflating injectable and topical dosing conventions applying an injectable milligram-target approach to a topical protocol that should use concentration-based formulation, or vice versa

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In animal studies spanning three decades, no lethal dose has been identified

The GST tag can easily be removed following purification by addition of a protease if a suitable protease-cleavage site has been inserted between the GST-tag and the protein of interest (which is usually included in many commercially available sources of GST-tagged plasmids)