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Methods Mol Biol 1303: 391403, 2016

Cells were plated on 48-well plates, coated with anti-CD3 (1 g ml 1 ) and anti-CD28 (10 g ml 1 ) antibodies, in RPMI medium supplemented with 10% FCS, penicillin, streptomycin, HEPES, glutamine, pyruvate, nonessential amino acids and -mercaptoethanol diluted with PBS (1:1) in the presence of IL-12 (3 ng ml 1 , Peprotech) (T H 1 conditions)

The advanced glycation end products (AGE) content in the plasma membrane of epithelial cells is directly increased by the conversion of the superoxide anion to hydrogen peroxide by SOD3, as seen in ( Figure 4 )

Experimental observations are intended exclusively for scientific exploration and hypothesis development within regulated research environments

Glutathione synthesis and its role in redox signaling