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Administering stem cell therapy during the initial stages of cartilage softening or fibrillation results in markedly superior outcomes: Early stem cell treatment activates cartilage repair pathways (SOX9, COL2A1, ACAN) , facilitating chondrocyte regeneration and halting degenerative cascades

The cold pressing method using hexane as a solvent does not involve heating or chemical treatment during oil extraction but gives low yields, whereas the Soxhlet extraction method using methanol as a solvent has low costs