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A system transport present in the inner mitochondrial membrane, that involves dicarboxylate and 2-oxoglutarate anion transporters, allows the passage of negatively charged GSH from the cytosol to the mitochondria

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2 Methods 2.1 Animals and procedures The animal experimental procedures were approved by the Animal Care and Use Committee of Oujiang Laboratory (SYXK2023-0047, OJLAB22121609), and were carried out in strict accordance with the National Institutes of Health Guidelines for the Use of Laboratory Animals

Excessive mtROS can lead to mitochondrial membrane depolarization, increase the mPTP, and facilitate the release of cytochrome c along with other pro-apoptotic factors (Spaggiari et al., 2024)