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Mechanism of Ferroptosis and its Role in Type 2 Diabetes Mellitus

The levels of aspartate aminotransferase (AST), blood urea nitrogen (BUN), alanine aminotransferase (ALT), and creatinine (CRE) were assessed utilizing a Cobas 6000 automatic biochemical analyzer (Roche, Switzerland)

Specifically, the production of excessive amounts of ethanol can result in growth and RPP inhibition (Ergn et al., 2019

Cell synchronization and flow cytometry analysis Cells were synchronized at the G1/early S-phase boundary using a double-thymidine (DT) block protocol: cells were cultured with 2 mM thymidine for 16 h, then released to thymidine-free medium for 8 h, and with 2 mM thymidine for another 16 h