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Electron paramagnetic resonance (EPR) spectroscopy was conducted using a Bruker A300-10/12 to verify the successful coordination of Cu 2+ with D-P

A 900 L aliquot of hypo-osmotic solution (prepared by dissolving 0.9 g fructose (Solarbio, #F8100, China) and 0.49 g sodium citrate (Solarbio, #S6100, China) in 100 mL deionized water) was pre-warmed at 37 C for 30 min in a constant temperature water bath

Barrier: It strengthens the skins protective layer (stimulates ceramide production)

Do not return the used solution to the bottle

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