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The measurement of absorbance at 490 nm was carried out with the employment of a microplate reader (680 Microplate Reader, Bio-Rad)

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We took advantage of quantitative 33 S-NMR to measure precisely the amount of sulfur incorporated, high-resolution liquid chromatography/mass spectrometry (LC-MS) to determine populations of isotopic species (isotopologues), and gas chromatography/mass spectrometry (GC-MS) for metabolic profiling

doi: 10.1016/j.algal.2019.101617 29