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However, the inevitable tissue disruption on extraction into aqueous buffer has the potential to generate artifacts arising from the interaction between a GST and a ligand usually found in a different compartment, or the generation of ligands formed through chemical- or enzyme-mediated oxidation during extraction

Primary bronchial epithelial cells, collection, storage, and use of tissue were performed in agreement with the Code for Proper Secondary Use of Human Tissue in the Netherlands ( The scientific board of the MPTC approved the use of materials for the present study under MPTC2010-019

Meanwhile, some limitations in the current study should also be considered

For instance, in certain scenarios, the absence of p53 suppresses the nuclear accumulation of dipeptidyl peptidase-4 (DPP4), fosters the formation of DPP4 and NOX1 complexes, and thereby intensifies lipid peroxidation, leading to ferroptosis [65]