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Each RT-PCR reaction consisted of 10 L of SYBR Green Supermix (Bio-Rad, iTaq Universal SYBR Green Supermix, 1725124), 7.6 L of distilled water, 0.4 L of primer mix, and 2 L of cDNA

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So, at least in these doses (more than twice the Institute of Medicine recommendation), there appears to be no additional health benefit