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However, benztropine use is associated with notable adverse effects, including cognitive impairment, dry mouth, urinary retention, constipation, and blurred vision

Among these, the NLRP3 inflammasome stands out as the most extensively investigated, chiefly responsible for the secretion of bioactive IL-1 and IL-18, consequently inducing inflammatory cell demise (162)

This allows for highly precise dosing and a faster onset of action, which is crucial for studies requiring exact control over compound levels and acute responses

Twenty-six hours post-infection, stable pools of cells were selected with 1.2 g/mL puromycin (Invitrogen, San Diego, USA) for MCF-7 cells and 1.4 g/mL puromycin for ZR-75-1 cells for 3 days or 100 g/mL hygromycin for MCF-7 and ZR-75-1 cells for 7 days before being used for subsequent experiments