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As a benchmark, Taxol, a well-established microtubule-stabilizing compound with known efficacy in SCI models, was included as a positive control 21

Oxidant formation can also arise via electron-leakage from mitochondria, from enzymes such as xanthine oxidase and lipoxygenases, and by uncoupling of enzymes such as endothelial and inducible nitric oxide synthases (NOS) (reviewed) 8

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These results further confirm that Cys180 is the critical residue for both caspase-3 recognition and MeCbl binding and thereby inhibition of GSDME cleavage and activation

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