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For starvation (B-27 deprivation) induction, standard NDM was replaced with nutrient-deprived medium (F12/DMEM:Neurobasal50:50, 1 N2 Supplement, 2 g/ml doxycycline hyclate)

Therefore, you dont need to be concerned about receiving too much B-12 because your body can simply eliminate the vitamin if its consumed in excess

J Pharmacokinet Pharmacodyn 48 , 321338 (2021)

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Positive feedback regulation of microglial glucose metabolism by histone H4 lysine 12 lactylation in Alzheimers disease