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We also added glutathione reductase Gor (1 M) and its co-substrate NADPH+H + (250 M), to ensure the retention of the reduced state of the exogenous glutathione (see Figure 6D for a schematic overview)

The tibial nerve was transected at 2 mm proximal to the sutures at which the first tracing was performed, and the proximal tibial nerve stump was soaked into 5% FG in saline for 60 min with Parafilm TM protection and the excessive tracer removed as described above

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Un complment contenant du glutathion oxyd (GSSG) oblige la cellule le rduire avant de pouvoir l'utiliser, ce qui consomme des ressources (NADPH) et limite l'intrt de la supplmentation