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1.2.2.3) Synthesis of (2S,3R,4S,5S,6S)-2-(4-(2-((tert-butoxycarbonyl)amino)-1 -(((4- nitrophenoxy)carbonyl)oxy)ethyl)-2-nitrophenoxy)-6- (methoxycarbonyl)tetrahydro-2H-pyran-3,4,5-triyl triacetate Previous compound (2S,3R,4S,5S,6S)-2-(4-(2-((tert-butoxycarbonyl)amino)-1 - hydroxyethyl)-2-nitrophenoxy)-6-(methoxycarbonyl)tetrahydro-2H-pyran-3,4,5-triyl triacetate (334 mg I 0.54 mmol) and 4-nitrophenyl chloroformate (219 mg 1 1.09 mmol) were dissolved in 6 mL of dry DCM at 0C

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Quantification of RUNX and BMP-2 RUNX (MBS2509507, MyBioSource, San Diego, USA) and BMP-2 (MBS701336, MyBioSource, San Diego, USA) levels were detected in aorta using rat specific enzyme-linked immunosorbent assay (ELISA) kits following the manufactures instruction