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After amplification, the lacZ fragment as well as the domesticated destination vector were sequentially digested with restriction enzymes BglII and NotI and ligated with T4 DNA ligase, leading to the finished and ready-to-use expression vector weird_plex

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These side effects are usually mild and decrease over time

For male C57BL/6 mice temperature was kept constant at 22 2 C, and relative humidity was maintained at 50 5%

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