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Ln a preferred embodiment, end-selection, as provided herein, can be used in order to facilitate a cloning step, such as a step of ligation to another polynucleotide (including ligation to a vector)

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In the United States, Europe and many other countries, Herceptin is approved in combination with chemotherapy for the adjuvant (post-surgery) treatment of HER2-positive early breast cancer, first-line treatment of advanced HER2-positive breast cancer and first-line treatment of advanced HER2-positive gastric cancer

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