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Protein labeling Cy5 (Cytiva, PA25031) or Alexa Fluor 488 (AF488) (Invitrogen, A10254) labeling of protein was done by incubating 200 M of SERF2 (T2C) or G3BP1 with a 10-molar excess of C 5 maleimide mono-reactive dye in 20 mM Tris-HCl pH 7.4 and 100 mM KCl buffer overnight, at 25 C (SERF2) or at 4 C (G3BP1), under continuous shaking at 300 rpm

However, light intensity had no effect on OsLIKE1 transcription in the TP309 variety (Fig

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Probiotics for gastrointestinal symptoms and quality of life in autism: a placebo-controlled pilot trial