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NIH mice (30 males and 30 females) were utilized to conduct bone marrow micronucleus assay after single intramuscular doses of 167, 333, and 667 mg/kg

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Kinetic studies also confirmed that SIRT4 was the first mammalian sirtuin discovered to be physiologically regulated by the [NAD + ]/[NADH] instead of by [NAD + ] (Pannek et al., 2017) as 30 M NADH, a physiological concentration found in the mitochondrial matrix (Sallin et al., 2018), inhibited SIRT4 activity