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Generation of ABCB1 knockout UO-31 cells CRISPR-mediated knockout of ABCB1 in UO-31 cells was achieved by co-transfecting cells with knockout and homology-directed repair vectors for ABCB1 (obtained from Santa Cruz Biotechnology, Dallas, TX) using Lipofectamine 2000 (Invitrogen) and subsequent selection with puromycin (3 g/mL)