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13 In the 1970s, Zubay demonstrated CFE from DNA templates, 14 thereby recapitulating combined transcription and translation outside living cells, enabling longer lasting reactions and incorporating genetic regulatory elements into CFE

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For instance, studies have demonstrated that chronic unpredictable mild stress (CUMS) in rodent models leads to transcriptional changes in key metabolic enzymes, thereby increasing the synthesis of hepatic fatty acids (278).Chronic stress in murine models has been linked to increased hepatic TGs and cholesterol levels, coupled with a decrease in total visceral fat (279)

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