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The sheared chromatin was diluted 10-fold with ChIP dilution buffer (20 mM Tris-HCl, pH 7.9, 2 mM EDTA, 150 mM NaCl, 0.5% Triton X-100, 1 mM DTT, and 1 protease inhibitor cocktail) for ChIP

It is possible to move from the paediatric definition of PFIC to a notion of cholestatic disorders encompassing both adult and paediatric forms of cholestasis