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Description

The enzyme glutathione reductase, which requires NADPH as a cofactor, recycles GSSG back to GSH, maintaining this ratio [2]

This is the "stockpile preparation"

The bacterial suspension and cell lines were incubated for 3.5 h at 37C in a humidified atmosphere at 5% CO 2

Thats not a coincidence

Our optimization of the plasmids and generation of thioredoxin fusion proteins where necessary eliminates much of the complexity associated with inclusion bodies and the resulting refolding processes otherwise required