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When fully dissociated, cells were centrifuged (230 g , 3 min) and the cell pellet resuspended and diluted to approximately 2.5E06 cells/mL in Recovery TM Cell Culture Freezing Medium (Gibco, #12648-010)

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The vaccine of claim 76, wherein the hMPV F polypeptide is a pre-fusion F polypeptide

This impaired FXR-TonEBP axis directly compromises renal concentrating ability [13, 30] while concurrent dysregulation of NOX2/gp91 reflects broader cellular stress response alterations

These systemic reactions are also typically short-lived