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In both coronary and cerebral vascular beds and in the aorta, chronic exposure to estrogen, either endogenous or by estrogen replacement in ovariectomized female rodents, decreases vascular tone in an endothelium dependent manner (Duckles and Krause, 2007

10.1172/JCI72151 39

7 Summary and outlook Current skin aging research encompasses various topics, including skin microbiota, the skin-gut axis, DNA methylation, miRNA, RNA sequencing, quantitative proteomics, and quantitative metabolite assays

Instrumentation All analyses were performed on a 1200 Series HPLC system (Agilent Technologies, Waldbronn, Germany) equipped with a quaternary pump, vacuum degasser, autosampler, module of temperature control, and spectrophotometric detector