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AGEs induced NOX2 leading to downregulation of Sirt1/Timp3 and finally resulting in activation of TNF- converting enzyme and inflammation

The mixture was turned into orange red by minimum addition of deionized water recorded with pH 6.2 after 12 h incubation

Plate was washed three time and chromogenic substrate 3,3,5,5-tetramethylbenzidine (TMB) which catalyzes by HRP to generate a blue color was added

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Radical S-adenosylmethionine maquette chemistry: Cx3Cx2C peptide coordinated redox active [4Fe-4S] clusters