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Following treatment cells were fixed in chilled methanol for 30 min at 4 C and kept in blocking buffer (2% FBS and 0.05% Tween-20 in PBS) 1 h at RT

A comprehensive analysis revealed that GPx1 is highly expressed in most cancers

This analysis was written to provide a detailed background about the current perceived complexity in model insects regarding RNAi and dsRNA signaling to researchers that use RNAi in silencing experiments as well as to scientists that develop RNAi-based methods for insect pest control