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Once the bacteria were normalized to OD 600 1 in LB chloramphenicol, an MOI of 100 was added to 0.5 10 6 primary murine macrophages from WT C57BL/6 or C57BL/6 deficient for phox ( gp91 phox-/- ) mice (The Jackson Laboratory, stock # 002365) that have been activated using 100ng/well recombinant murine IFN- (Peprotech, #315-05) for 18 hours

Purification of the GST-fusion polypeptides In a 2 ml sterile Eppendorf tube, 1000 L (bed volume 800 L) of Glutathione S-sepharose beads were centrifuged at 375 g at 4 C/1 min

However, the enzymes downstream to MAOB in this pathway remain unidentified

Mingay M, Chaturvedi A, Bilenky M, Cao Q, Jackson L, Hui T, et al

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