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Mass spectrometry analyses of the immuno-enriched Flag-tagged-G6PD revealed Tyr phosphorylation at sites 249 and 322 (Y249/Y322) (Figure S2C)

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Quality control of inductively coupled plasmatriple quadrupole mass spectrometer measurements was ensured through a combination of repeat measurements of: Acid blanks A quality control standard made from 1,000 mg l 1 High-Purity Standards multi-element standard solutions A tenfold dilution of the CRM-TMDW (trace metal drinking water) from High-Purity Standards Analyte measurements were normalized to the internal standard Iridium ( 193 Ir) to account for instrument drift and matrix effects, and measurements were subsequently blank corrected by removing the average analyte intensity of repeat blank measurements