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Classical apoptosis remains the dominant mode of follicular loss: double-strand breaks trigger ATM/ATR-p53 signalling, up-regulating BAX/BAK and prompting mitochondrial outer-membrane permeabilisation with cytochrome-c release [78,79,80,81]

After 2 days, the cells were first treated with the indicated compounds for 4 h and then with 30 or 100 nM MZ1 for an additional 2 h

The most affected brain regions containing a high amount of neuromelanin and a high concentration of copper are the locus coeruleus and substantia nigra (Davies et al., 2013[106])