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Reverse transcription quantitative PCR (RT-qPCR) reactions were run in 12 l with PowerUp SYBR Green Master Mix (Applied Biosystems) for 40 cycles

AngelucciFOlivieroAPilatoFSaturnoEDileoneMVersaceVet al

This experiment was repeated another time using the same reaction conditions and an additional time containing 2% Casamino: however, no growth was observed

Table 2 summarizes the bioavailability challenges faced by major plant antioxidant classes and the corresponding enhancement strategies that have proven most effective in improving their therapeutic potential