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It is usually given either orally 2 h before the cyclophosphamide or intravenously at the same time to cover the period of maximum urinary excretion of toxic cyclophosphamide metabolites

346,347,348 These techniques have been combined with single-cell transcriptome to study gene regulatory profiles and analyze cell heterogeneity

IV therapy is significantly more effective, providing 90-100% bioavailability compared to only 10-50% for oral supplements

Int J Mol Sci, 2020, 22(1)

However, there are several limitations to this system, such as protein expression in inclusion bodies, incorrect folding, and inactive protein production caused by mispairing of disulfide bonds [25]